慢性束缚应激对大鼠脑内Fas、FasL含量的影响

Effects of Chronic Immobilization Stress on the Expression of Fas/FasL in the Brain of Rats

为探讨慢性束缚应激对大鼠脑内不同部位Fas/FasL系统表达的不同影响。将24只雄性SD大鼠随机分为束缚应激组、装置对照组和正常对照组（n=8）。分别对三组大鼠给予相应的干预14天，用Western-blotting方法测定应激后大鼠大脑前脑皮质、内嗅皮质以及海马区域Fas、FasL蛋白含量。结果表明，应激后各脑区三组大鼠Fas含量差异具有统计学意义（p < 0.01），束缚应激组明显高于正常对照组或装置对照组（p < 0.01）。在海马区域，三组大鼠FasL含量差异具有统计学意义（p < 0.01），束缚应激组大鼠FasL蛋白水平显著高于正常对照组或装置对照组(p < 0.01)。三组大鼠自身大脑前脑皮质、内嗅皮质以及海马Fas含量的差异比较均无统计学意义( p > 0.05) 。束缚应激组大鼠海马的FasL含量高于前脑皮质和内嗅皮质(p < 0.05)；正常对照组或装置对照组自身不同脑区间FasL含量的变化无统计学意义。提示慢性束缚应激能诱导大鼠脑内Fas/FasL系统表达水平的改变，对不同的脑区，其影响程度明显不同。

Currently， there exists a substantial body of evidence suggesting that chronic stress can increase susceptibility to diseases such as depression and posttraumatic stress disorder. Chronic stress induces neuronal atrophy and death in the cortex as well as in the hippocampus. Proto-oncogene protein kinase B (PKB)， also known as Akt， plays a central role in a signaling pathway whose various components have been linked to cellular survival. In recent years， Akt as well as one of its downstream targe