脂多糖免疫激活导致抑郁性行为的动物研究：剂量和时程效应

Animal Research on Immunity Activation-Induced Depressive-Like Behavior：Doses and Time Effects

“抑郁症细胞因子假说”的提出为抑郁障碍的病因学研究提供了一个新的方向，为了探讨脂多糖(lipopolysaccharide， LPS)诱导的免疫激活与抑郁性行为产生之间的关系，本研究采用50只SD大鼠随机分为五组LPS400，LPS200，LPS50，LPS10，LPS0，分别于实验期第0天和第3天注入LPS400µg/㎏，200µg/㎏，50µg/㎏10µg/㎏和生理盐水。以糖精水偏爱，旷场行为和高架十字迷宫评定大鼠LPS注射后2h，24h，48h的行为变化。结果显示一次LSP注射后2h，LPS50，LPS200，LPS400组动物与生理盐水组动物相比较，其糖精水偏爱分数(p<0.01)和直立行为(p<0.01)以及高架十字迷宫中的闭合臂进入次数(p<0.01)和开放臂进入次数显着下降(p

The “cytokine theory of depression” indicated that cytokines induced by immunity activity are not only immunity mediators but also play an important role in the mechanism of depressive - -like behaviors. The administration of lipopolysaccharide (LPS)， which is a product of the cell wall of Gram – negative bacteria， is known to activate immune functions and induce the release of several cytokines both in the periphery， and the brain such as frontal cortex， hippocampus， and hypothalamus that are c