慢性心理应激对大鼠淋巴细胞热休克蛋白70表达和凋亡的影响,ERICDATA高等教育知識庫
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篇名
慢性心理应激对大鼠淋巴细胞热休克蛋白70表达和凋亡的影响
並列篇名
Effects of Psychological Stress on the Hsp70 Expression and Apoptosis of the Peripheral Blood Lymphocytes in Rats
作者 颜军陈爱国胡茂志
中文摘要
为了探讨慢性心理应激对热休克蛋白70(Hsp70)、淋巴细胞的凋亡及其两者之间关系的影响,将实验SD大鼠分为对照组、中等强度心理应激组和高强度心理应激组。分别对三组大鼠给予相应的干预3周,采用流式细胞仪定量检测各组大鼠外周血淋巴细胞Hsp70的表达水平和凋亡量。结果发现,与对照组比较,中等和高强度心理应激组淋巴细胞内Hsp70表达水平显著增加;中等强度心理应激组凋亡量增加但没有出现统计学意义,高强度心理应激组淋巴细胞凋亡显著增加;中等强度心理应激组的淋巴细胞Hsp70表达与凋亡量变化成显著正相关,高强度心理应激组两者变化成不显著正相关。研究结果提示心理应激能诱发淋巴细胞 Hsp70表达和凋亡升高,且随心理应激强度增加,Hsp70表达升高趋势减缓、凋亡量增加加剧,最终造成机体免疫损伤加深。
英文摘要
Heat shock proteins (HSP) represent a very conservative family of cytoprotective proteins that are specifically induced in response to several environmental stresses at the cellular level (heat shock, cellular energy depletion, oxidative stress, or inflammation). Among these proteins, the inducible form of the 70 kDa family, i.e., Hsp72, plays an important role in cytoprotection by preventing the abnormal folding of newly synthesized polypeptides, or by assisting in the repair of damaged proteins or in the degradation of irreversibly damaged proteins. An adapted stress response in terms of transcription and translation of Hsp70 is necessary to ensure cell survival under stressful conditions. Lymphocytes apoptosis plays an important role in the immune function. It is now well documented that acute psychological stress, particularly when it reaches high intensity, leads to high Hsp70 expression and lymphocytes apoptosis. Relatively fewer studies have been conducted on the effects induced by chronic psychological stress on Hsp70 expression in lymphocytes, and the relation between Hsp70 expression and lymphocytes apoptosis. The aim of this study is to investigate the effect of psychological stress on Hsp70 expression, lymphocytes apoptosis, and the relationship between them.Twenty-four SD rats were randomly divided into three groups of eight as follows: control group (C), high intensity psychological stress group (H), and moderate intensity psychological stress group (M). A flow cytometer detected the extent of apoptosis and hsp70 expression in the peripheral blood lymphocytes of rats under psychological stress of varying intensity. One-way ANOVA and correlation analysis were used for the statistical analysis.In comparison with group C, the Hsp70 expression in lymphocytes groups H and M was significantly higher. The extent of apoptosis in group H increased significantly, while the extent of apoptosis in group M did increase, but not significantly. The positive correlation between the changes of Hsp70 expression and the lymphocytes apoptosis in group M was significant, while the correlation for group H was positive, but not significantly.This study suggested that chronic psychological stress, especially that of high intensity, increased the Hsp70 expression and the lymphocytes apoptosis in peripheral blood lymphocytes. Furthermore, as the intensity of psychological stress increases, the Hsp70 expression in lymphocytes increased slowly while the extent of apoptosis increased rapidly. As a result, the immune function kept deteriorating.
起訖頁 717-722
關鍵詞 细胞凋亡热休克蛋白70淋巴细胞慢性心理应激apoptosisHsp70lymphocytespsychological stress
刊名 心理學報  
期數 200806 (40:6期)
出版單位 中國科學院心理研究所;中國心理學會
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