雄性HSF1基因缺陷小鼠的行为改变

Behavioral Characterization of HSF1 Knockout Male Mice

为研究HSF1基因缺陷小鼠的行为特征，探索HSF1基因在小鼠行为表现中的作用。选取6~7个月大雄性HSF1基因缺陷小鼠39只及野生型小鼠36只进行情绪性评分、旷场实验、高架十字迷宫实验、简易迷津实验、T-CAT实验、独木桥实验和悬挂实验以观察其情绪性唤醒水平、焦虑水平、探索行为、工作记忆能力和运动能力。结果表明HSF1基因缺陷小鼠的情绪唤醒水平和焦虑水平较低、探索行为减少、T-CAT中转换率较低，提示小鼠的情绪、探索动机和工作记忆受HSF1基因的调控。

Past research has indicated that the heat shock factor (HSF) family mediates the induction of heat shock proteins in response to various types of stress. HSF1 is the primary factor controlling the stress-inducible expression of HSP genes. The aim of the present study was to examine behavioral phenotype of HSF1 knockout male mice (HSF1 ?/? mice).Methods The behavior of HSF1 ?/? mice was compared to those of a wild-type control in a series of experimental tests and conditions， including: (1) emotionality rating scale， (2) elevated plus maze， (3) open field test， (4) simple maze test， (5) T-maze continuous alternation task (T-CAT)， (6) bridge walking， and (7) wire suspension test.Results We find that HSF1 ?/? mice: (1) had significantly lower emotionality rating scores， (2) spent a greater amount of time in the open arms of the elevated plus maze， (3) were less active in both open field test and simple maze test， and (4) showed impaired working memory as assessed by the T-CAT. There were no significant effects of genotype on the scores from the bridge walking and wire suspension test.Conclusions These findings suggest that HSF1 ?/? mices possess impaired motor exploratory motivation， and HSF1 ?/? mice possess lower anxiety levels. At the same time， HSF -/- mices that have impaired working memory do not appear to affect locomotive ability.